Preparation of pure microbiological samples for pyrolysis of gas-liquid chromatography studies.
نویسندگان
چکیده
Bacterial samples were prepared for pyrolysis gas-liquid chromatography using cells grown on membrane filters. Pyrochromatograms were reproducible when cells harvested from the filters were pyrolyzed without being washed.
منابع مشابه
Characterization of the Products Obtained in Coal Pyrolysis: A Case study of Some Pakistani Coals
Thermal decomposition of two coal samples obtained from Top Seam Sibi (TSS) and Sore Range Quetta (SRQ) coal fields in their raw and acid washed forms were carried out in an open type tubular pyrolyzer coupled to a gas chromatograph with FID. Elemental analyses were carried out with Scanning Electron Microscope-Energy Dispersive X-rays (SEM-EDX) analyzer. Effect of inherent mineral contents...
متن کاملRelative Determination Approach to the Metabolites of Protoberberine Alkaloids in Rat Urine by Liquid Chromatography Tandem Mass Spectrometry for the Comparative Studies on Rhizome coptidis and Zuojinwan Preparation
The lack of authentic standards has limited the quantitative analysis of herbal drugs in biological samples. The present work demonstrated a practicable strategy for the assay of herbs and their metabolites independent of authentic standards. A liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS) method for the qualitative and quantitative determination of the metabolites...
متن کاملDeasphalting of Olefin Pyrolysis Fuel Oil by Combination of Chemical–Physical Methods
This work investigates the effect of different conditions of PFO thermal cracking from two PFO samples on liquid, solid and gas product yield and asphaltene removal efficiency in a new designed experimental setup. No need to use a catalyst, simple operating system and experiment conditions, the ability to use water as a cheap carrier gas and high asphaltene extraction efficiency without the use...
متن کاملDifferentiation of selected Enterobacteriaceae by pyrolysis-gas-liquid chromatography.
Pyrolysis-gas-liquid chromatography was used to differentiate selected species of Enterobacteriaceae. Individual cultures of Salmonella typhi, Hafnia alvei, and Proteus vulgaris, and 12 strains of Yersinia enterocolitica were grown in nutrient broth. After harvest and lyophilization, the bacterial samples were pyrolyzed at 900 degrees C, and their volatile fractions were separated on a 50-m cap...
متن کاملDetermination of Organic Sulfur Contaminants Using Hollow Fibre-protected Liquid-phase Micro-extraction Coupled with Gas Chromatography
A simple and solvent-minimized sample preparation technique based on hollow fibre-protected liquid-phase micro-extraction has been developed for extraction of fifteen organic sulphur compounds from aqueous samples. The analysis of the extracted them was performed by gas chromatography equipped with mass spectrometry and/or flame photometric detectors. 3.3 µL of organic solvent located in the lu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Applied and environmental microbiology
دوره 32 2 شماره
صفحات -
تاریخ انتشار 1976